https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29909 P < 0.001) were found between ALDH expression and various motility parameters of stallion spermatozoa including the percentage of progressive (r = 0.79) and rapidly motile (r = 0.79) spermatozoa, whereas repeated measurements over 24 h revealed highly significant correlations among progressive motility loss, 4HNE accumulation, and ALDH expression (P ≤ 0.001). ALDH inhibition resulted in a spontaneous increase in 4HNE levels in viable cells (21.1 ± 5.8% vs. 42.6 ± 5.2%; P ≤ 0.05) and a corresponding decrease in total motility (41.7 ± 6.2% vs. 6.4 ± 2.6%; P ≤ 0.001) and progressive motility (17.0 ± 4.1% vs. 0.7 ± 0.4%; P ≤ 0.001) of stallion spermatozoa over 24 h. Similarly, inhibition of ALDH in 4HNE-challenged spermatozoa significantly reduced total motility over 4 h (35.4 ± 9.7% vs. 15.3 ± 5.1%, respectively; P ≤ 0.05). This study contributes valuable information about the role of the ALDH enzymes in the maintenance of stallion sperm functionality, with potential diagnostic and in vitro applications for assisted reproductive technologies.]]> Wed 11 Apr 2018 16:45:10 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27549 Wed 11 Apr 2018 14:20:32 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30037 Wed 11 Apr 2018 13:40:38 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34663 Wed 10 Apr 2019 13:35:43 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54790 Tue 12 Mar 2024 14:35:02 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42127 P < 0.001). Furthermore, the deleterious effects of GSH depletion using menadione and 1,3 dimethoxy 1,4, naphtoquinone (DMNQ) were able to be prevented by the addition of cysteine, but no other antioxidant. Pre-incubation with cysteine prevented menadione and DMNQ induced damage to sperm membranes after 1 h (P < 0.001; P < 0.05) and after 3 h of incubation (P < 0.001, P < 0.05). Pre-incubation with cysteine ameliorated both the menadione- and DMNQ-induced increase in 4-hydroxynonenal (P < 0.001). As cysteine is a precursor of GSH, we hypothesized that stallion spermatozoa are able to synthesize this tripeptide using exogenous cysteine. To test this hypothesis, we investigated the presence of two enzymes required to synthesize GSH (GSH and GCLC) and using western blotting and immunocytochemistry we detected both enzymes in stallion spermatozoa. The inhibition of GCLC reduced the recovery of GSH by addition of cysteine after depletion, suggesting that stallion spermatozoa may use exogenous cysteine to regulate GSH. Other findings supporting this hypothesis were changes in sperm functionality after BSO treatment and changes in GSH and GSSG validated using HPLC-MS, showing that BSO prevented the increase in GSH in the presence of cysteine, although important stallion to stallion variability occurred and suggested differences in expression of glutamate cysteine ligase. Mean concentration of GSH in stallion spermatozoa was 8.2 ± 2.1 μM/10⁹ spermatozoa, well above the nanomolar ranges per billion spermatozoa reported for other mammals.]]> Thu 25 Aug 2022 12:01:39 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52633 Thu 19 Oct 2023 15:11:54 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19260 Sat 24 Mar 2018 08:06:38 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20539 Sat 24 Mar 2018 08:02:43 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29650 Sat 24 Mar 2018 07:41:51 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26749 Sat 24 Mar 2018 07:24:44 AEDT ]]>